%0 Journal Article
%T Mass Culture and Purification of Vero-based Influenza Virus<br>Vero 细胞流感病毒的大规模培养和纯化
%A 何春艳
%A 陈则
%A 吴书军
%A 唐丽丽
%A 秦洁
%J 中国生物工程杂志
%D 2008
%I 
%X Objective Establish mass-scale purification technology of cell-derived influenza virus. Methods A microcarrier-based process was used to produce human influenza virus in serum free-adapted Vero cells. The virus was purified in a sequence of downstream processing steps including inactivation, clarification, anion exchange chromatography, affinity chromatography and size-exclusion chromatography. Results The recovery of HA reached 102%. 95.3% total protein, including 99.77% host cell protein, and 99.99% host cell DNA were removed during downstream processing. Conclusion This research provides a high effective purification technology for cell-derived influenza virus.
%K Vero细胞
%K 流感病毒
%K 纯化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=0F84F8362E0BC761A0A5A2943CF26F12&yid=67289AFF6305E306&vid=D3E34374A0D77D7F&iid=708DD6B15D2464E8&sid=A04140E723CB732E&eid=B91E8C6D6FE990DB&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=8