%0 Journal Article
%T The Synthesis of Novel Protein Modification Reagent and its Application of Modifying Bovine Hemoglobin<br>新型蛋白质修饰剂的合成及修饰牛血红蛋白的初步研究
%A XING Wen-chao
%A YUN Qiang
%A SU Zhi-guo
%A CAO Xu
%A <br>邢文超
%A 曹旭
%A 贠强
%A 苏志国
%J 中国生物工程杂志
%D 2006
%I 
%X A novel tetrafunctional reagent for protein modification was successfully synthesized by L-glutamic acid and adipic acid, and it was characterized by H1-NMR and FTIR. The new reagent was then applied to modify bovine hemoglobin, and the preliminary properties of modified protein were characterized by HPLC, SDS-PAGE and Hemox Analyzer. The results showed that the intra-molecular and inter-molecular cross-linking of bovine hemoglobin was formed simultaneously by using the new reagent, and the modified hemoglobin performed well for O2 delivery (P50: 21.7 mmHg, Hill coefficient: 2.01). Therefore, the new cross-linking reagent is promising in development of stroma-free hemoglobin based artificial blood substitutes.
%K Bovine hemoglobin Chemical modification Blood substitutes<br>血红蛋白
%K 蛋白质化学修饰
%K 血液代用品
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=3494C67431665248&yid=37904DC365DD7266&vid=96C778EE049EE47D&iid=38B194292C032A66&sid=B31275AF3241DB2D&eid=F3090AE9B60B7ED1&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=1&reference_num=15