%0 Journal Article
%T Disruption of hom gene encoding for homoserine dehydrogenase of Corynebacterium glutamicum<br>谷氨酸棒杆菌高丝氨酸脱氢酶编码基因hom的敲除
%A RAO Zhi-ming
%A ZHANG Jun-sheng
%A SHEN Wei
%A FANG Hui-ying
%A ZHUGE Jian
%A <br>饶志明
%A 张君胜
%A 沈微
%A 方慧英
%A 诸葛健
%J 中国生物工程杂志
%D 2007
%I 
%X The hom gene encoding for homoserine dehydrogenase was amplified from the genomic DNA of Corynebacterium glutamicum ATCC 13032.After the kanamycin-resistant gene(Km)cassette from plasmid pET28a was inserted into the center of hom,the hom::Km cassette was then electroporated into the competent cell of C.glutamicum ATCC 13032.And kanamycin-resistant clones were obtained.PCR was performed to confirm whether the Km gene was integrated into the hom gene of these clones and the recombinant strains of hom-disrupted were screened out.Fermentation results showed that the lysine yield of the hom-disrupted strain C.g-hom::Km-8 reached 4.7 g/L,which was 6.7 times that of C.glutamicum ATCC 13032.
%K Lysine Homoserine dehydrogenase-disruption Corynebacterium glutamicum<br>赖氨酸
%K 高丝氨酸脱氢酶缺陷
%K 谷氨酸棒杆菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=11D981E2B0B5CEBE&yid=A732AF04DDA03BB3&vid=DB817633AA4F79B9&iid=CA4FD0336C81A37A&sid=6AC2A205FBB0EF23&eid=E84BBBDDD74F497C&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=5