%0 Journal Article
%T The Study on Somatic Cell Cloned Cattle in China<br>我国体细胞克隆牛的研究进展
%A CHENG Jin-hua
%A ZHU Hua-bin
%A DAI Yun-ping
%A SUN Xiu-zhu
%A WANG Dong
%A HAO Hai-sheng
%A <br>程金华
%A 朱化彬
%A 戴蕴平
%A 孙秀柱
%A 王栋
%A 郝海生
%J 中国生物工程杂志
%D 2006
%I 
%X Because of the important role of cloning technique in husbandry production, therapy of the disease, study of the basic theory and the conservation of animal resource, the efficiency of the cloning technique was becoming the present focus of the study. The factors related to the cloning technique were reviewed. The effect of different cell lines on the efficiency of somatic cell cloned cattle were different, the blastocyst rate, pregnant rate and calving rate of fetal cells and the cumulus cells were higher than ones of adult fibroblast. The study about refrigerant conservation of cloned embryos and oocyte indicated that the vitrification refrigeration method could be used to freeze the oocyte and the cloned cattle embryos. The new progress of the study on mammalian cell clone and the efficiency of somatic cell clone were also discussed, and the urgent problem in somatic cell clone was presented.
%K Cattle Somatic cell nuclear transfer Efficiency<br>牛
%K 体细胞克隆
%K 核移植
%K 效率
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=031E5932282AA15F&yid=37904DC365DD7266&vid=96C778EE049EE47D&iid=F3090AE9B60B7ED1&sid=80A07035DF96B0C4&eid=E203FB1A272C9DD2&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=32