%0 Journal Article
%T Effects of the culture method on the construction of dermal substitutes in vitro<br>培养方式对真皮组织体外构建的影响
%A HE Yan-na
%A DING Chun-mei
%A JIANG Hua
%A ZHOU Yan
%A TAN Wen-song
%A <br>何燕娜
%A 丁春梅
%A 江华
%A 周燕
%A 谭文松
%J 中国生物工程杂志
%D 2007
%I 
%X Culture environment is the key factor in the construction of dermal skin. This study reports on the effects of culture methods on the construction of dermal substitutes using collagen-chitosan sponges in vitro. Through comparing the static culture and spinner flask culture methods, and the spinner flask culture with different stir speeds 10 r/min, 40 r/min and 80 r/min, the effects of the culture environment on the cells proliferation, cells distribution within scaffold and cells metabolism had been studied. A greater cell density was obtained with spinner flask culture versus static culture, especially, the 80 r/min spinner flask culture shows the obvious advantage with the greatest cell density and specific growth rate. The stirred culture of fibroblast-seeded sponges in spinner flasks resulted in a more uniform cell distribution compared to static culture. The dermal substitutes obtained from the 80 r/min spinner flask culture have greater cell density and more uniform distribution within scaffold than the 10 r/min and 40 r/min spinner flaks. In summary, the spinner flasks culture with proper stir speed shows promise for the in vitro construction of dermal substitutes.
%K Tissue engineering Dermal Spinner flask<br>组织工程
%K 真皮
%K 转瓶培养
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=41CEFB8691AEC774&yid=A732AF04DDA03BB3&vid=DB817633AA4F79B9&iid=94C357A881DFC066&sid=80A07035DF96B0C4&eid=E203FB1A272C9DD2&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=1&reference_num=16