%0 Journal Article
%T A peptide with ability to modify nNOS activity<br>噬菌体展示筛选得到一种能修饰nNOS活性的多肽
%A PAN Ming-Yang
%A GONG Zi-li
%A XU Hong
%A PAN Ying
%A ZHU Min-sheng
%A CHEN Hua-qun
%A <br>潘明阳
%A 龚自力
%A 徐洪
%A 潘英
%A 朱敏生
%A 陈华群
%J 中国生物工程杂志
%D 2006
%I 
%X Change in the neuronal nitric oxide synthase (nNOS) is a key factor in pathogenesis of a number of clinical disorders. In order to find specific molecule with ability to modify nNOS activity, the nNOS protein fragment contains the sequence around Calmadulin binding region was expressed in E. Coli. The purified recombinant protein was used as target protein and a 12-mer phage-displayed peptide library was screened to identify potential nNOS inhibitor. 10 phage clones were shown to specifically inhibit nNOS activity. DNA sequencing of the clones revealed a common amino acid sequence KPHHHPMPPQVS, this sequence was named NPI. The NPI was synthesized and showed inhibition effect on activity of freshly prepared nNOS from mice brain (inhibition rate is 25% ~ 35%). Howerever, to nNOS that lost part activity , the peptide recovered the activity in a dose-dependent fashion. Our results suggest an approach to develop new drugs to treat diseases based on nNOS disorder and understanding the process of nNOS in vivo.
%K nNOS<br>噬菌体展示库
%K 活性修饰
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=933324A11FC94B08&yid=37904DC365DD7266&vid=96C778EE049EE47D&iid=94C357A881DFC066&sid=1AE5323881A5ECDC&eid=B6DA1AC076E37400&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=14