%0 Journal Article %T Research Progress on Determination of Cellulase Activity and Gene Expression by Biosensor
生物传感器检测纤维素酶活性及基因表达的研究进展 %A YIN Juan %A YUAN Xing-zhong %A TANG Lin %A
尹娟 %A 袁兴中 %A 汤琳 %J 中国生物工程杂志 %D 2009 %I %X Cellulase system contains a series of complex components. There are still some problems remained unclear in cellulase and its mechanism of hydrolyzing lignocellulosic materials and its hydrolysis kinetics, so profound study is needed. The rapid development of many kinds of new interdiscipline such as biochemistry, molecular biology and gene engineering, has further clarified the structure and function of cellulase, and the relationshi Pof its gene expression and regulation, and furthermore resulted in derivative study methods about cellulase in more aspects. Cellulase system components according to synergistic catalytic mode and the sequence of the homology amino acids similarity, summarizes traditional detection methods of enzyme components, and emphasizes on research progress of various biosensors applied in detection of cellulase activity and gene expression was introduced. %K cellulase %K biosensor %K enzyme activity %K gene expression
纤维素 %K 酶生物传感器 %K 活性 %K 基因表达 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=CCD83C1A4ED48D91E7CCACF6E26032DE&yid=DE12191FBD62783C&vid=771469D9D58C34FF&iid=CA4FD0336C81A37A&sid=7AA74D31F1FF2DCE&eid=39EEF47180459690&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=35