%0 Journal Article
%T Progress in Construction Methods of Diverse Mutated Gene Libraries for Protein<br>蛋白质突变体基因库构建方法的研究进展
%A ZHANG Xiu-yan
%A HE Guo-qing
%A <br>张秀艳
%A 何国庆
%J 中国生物工程杂志
%D 2006
%I 
%X Directed evolution has been proven to be an effective method for proteins engineering. In the past few years, many new protocols for directed evolution were developed on the basis of oligonucleotide -directed random mutation, error-prone PCR and DNA shuffling. These new methods and their characteristics were summarized to provide a reference for choosing an approach to modify specific protein properties. The recent researches indicated that the combined directed evolution, coupling the directed evolution with rational design and computational design approaches respectively, is becoming increasingly important in protein engineering and is becoming the new direction for directed evolution.
%K Directed evolution Gene libraries Protein engineering Computational design approaches Rational design<br>定向进化
%K 基因文库
%K 蛋白质工程
%K 计算设计方法
%K 理性设计
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=36C49FD4D550832A&yid=37904DC365DD7266&vid=96C778EE049EE47D&iid=F3090AE9B60B7ED1&sid=771152D1ADC1C0EB&eid=014B591DF029732F&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=2&reference_num=42