%0 Journal Article
%T Prokaryotic expression and functional study of a novel recombinant human-derived collagen<br>新型促细胞粘附重组人源性胶原的原核表达及功能性研究
%A Kun Hu Fu-zhai Cui Li Xu Heng-liang Wang Liu-yu Huang
%A <br>胡堃
%A 崔福斋
%A 徐莉
%A 王恒樑
%A 黄留玉
%J 中国生物工程杂志
%D 2007
%I 
%X The DNA sequences, which originated from gene COL1A1, optimized for E.coli codon usage and contained cell adhesion domains of GER units, was amplified by RT-PCR technology from human tissue. The recombinant expression vector pET28a( )-COL encoded the recombinant human-derived collagen (RHDC) was constructed and transformed host E.coli strain BL21(DE3)pLysS. The RHDC production was induced by the addition of IPTG and approximately 40% of the total strain proteins. RHDC could form a triple-helical structure suggested by the SDS-PAGE and Western blot analysis. Cell adhesion assay indicated that RHDC had high cell adhesive character, which makes it suitable for use as biomaterials.
%K Recombinant human-derived collagen Cell adhesive Prokaryotic expression Functional study<br>重组人源性胶原
%K 细胞粘附
%K 原核表达
%K 功能研究
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=7D9DC226D1E45EA0&yid=A732AF04DDA03BB3&vid=DB817633AA4F79B9&iid=5D311CA918CA9A03&sid=CA4FD0336C81A37A&eid=B31275AF3241DB2D&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=26