%0 Journal Article
%T Generation of a recombinant fowlpox virus co-expressing Foot-and-Mouth Disease virus P1-2A gene and porcine interleukin 18<br>共表达O型口蹄疫病毒P1-2A基因和猪白细胞介素18的重组鸡痘病毒的构建
%A LIU Hui-juan
%A JIN Ning-yi
%A MA Ming-xiao
%A JIN Ming-lan
%A ZHANG Lin
%A LI Xu
%A JI Yue
%A LU Hui-jun
%A JIN Kuo-shi
%A <br>刘慧娟
%A 金宁一
%A 马鸣潇
%A 金明兰
%A 张林
%A 李旭
%A 计越
%A 鲁会军
%A 金扩世
%J 中国生物工程杂志
%D 2006
%I 
%X Objective:In order to prevent the O-FMDV, a recombinant FPV which can co-expressing foot-and-mouth disease virus P1-2A gene and porcine interleukin -18 as the vaccine was constructed and screened. Methods:Two primers for amplifying the O-FMDV P1-2A gene were desigined:the Kozak sequence was inserted into the upstream primer;the linker was inserted into the downstream primer. Then pIL-18 gene was connected to P1-2A gene in order to construct the expression gene box, which named as P1-2A-IL-18. The gene box was inserted into the FPV transfer vector pUTA-16-LacZ-3C to construct recombinant transfer vector plasmid pUTAL-3C-P1-2A-IL-18.The recombinant transfer vector plasmid was cotransfected with 282E4 fowlpox viruses into the CEF. The recombinant fowlpox viruses-3C-P1-2A-IL-18 (rFPV-3C- P1-2A-IL-18) was selected 3 passages by culturing in CEF with MEM medirum containing BrdU. The selected viruses were plaque-purified in CEF without BrdU. After RT-PCR and IFA analysis, the recombinant FPVs expressing gene box P1-2A-IL-18 was obtained. Results:The rFPV-3C-P1-2A-IL-18 strains could express 3C-P1-2A gene and pIL-18 gene correctly. Conclusion:It indicated that a recombinant fowlpox viruses rFPV-3C-P1-2A-IL-18 which can co-expressing foot-and-mouth disease virus P1-2A gene and porcine interleukin 18 was successfully obtained.
%K FMDV Fowlpox virus vector P1-2A gene IL-18 gene 3C gene<br>口蹄疫病毒
%K 鸡痘病毒活载体
%K P1-2A基因
%K IL-18基因
%K 3C基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=0AA48E78DD2666D1&yid=37904DC365DD7266&vid=96C778EE049EE47D&iid=9CF7A0430CBB2DFD&sid=A04140E723CB732E&eid=EA389574707BDED3&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=1&reference_num=7