%0 Journal Article
%T Detection of Drug-resistant Mutations in HIV-1 RT Gene Using Magnetic Beads Associated DNA Isolation Technology<br>磁珠分离DNA技术检测HIV-1逆转录酶基因耐药性突变
%A CHENG Shao-hui
%A HE Hong-qiu
%A LIU Bin
%A CHEN Wei-zu
%A WANG Cun-xin
%A <br>程绍辉
%A 何红秋
%A 刘斌
%A 陈慰祖
%A 王存新
%J 中国生物工程杂志
%D 2008
%I 
%X In recent years, the emergence of drug-resistant RT gene mutations in HIV-1 strains has become the major reason for AIDS treatment failure. The detection of drug-resistant mutations is important for the treatment choice. In this work, a novel assay for the detection of HIV-1 RT drug-resistant mutations was developed. Drug-resistant and wild strains of HIV-1 B subtype were investigated based on Two-Step MS-PCR. Magnetic beads associated DNA isolation technology and ELISA were employed in this assay to detect point mutations including Y181C and T215F. As a result, this novel assay gave a good P/N ratio and as less as about 5% of mutant type template could be detected, indicating that this assay is highly sensitive and specific. The assay can be completed in relatively less time consumption and applied in high-throughput screening. We suggest that it will be used in clinic assay for the detection of HIV-1 drug-resistant mutations as well as other point mutations.
%K 逆转录酶
%K 耐药性突变
%K 磁珠
%K ELISA
%K MS-PCR
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=B996DF2375190B74FA44131F1E9A1BF3&yid=67289AFF6305E306&vid=D3E34374A0D77D7F&iid=5D311CA918CA9A03&sid=03F1579EF92A5A32&eid=91C9056D8E8856E0&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=1&reference_num=13