%0 Journal Article
%T Effects of Vitamins on the Direct Fermentative Production of L-serine in Corynebacterium glutamicum SYPS-062<br>维生素对谷氨酸棒杆菌SYPS-062直接发酵合成L-丝氨酸的影响
%A ZHANG Xiao-juan
%A DOU Wen-fang
%A XU Hong-yu
%A XU Zheng-hong
%A <br>张晓娟
%A 窦文芳
%A 许泓瑜
%A 许正宏
%J 中国生物工程杂志
%D 2007
%I 
%X The effects of six vitamins on a novel isolate,Corynebacterium glutamicum SYPS-062,which could directly produce L-serine from carbohydrates were investigated.The results show that all these tested vitamins-VB1,biotin,VB2,VB6,folate and VB12 could enhance the growth of C.glutamicum SYPS-062 and improve the production of L-serine.However,the mechanisms of their metabolic regulations were different.Although VB1,biotin and VB6 could enhance the L-serine degradation,the significant increasing of biomass induced the increment of L-serine accumulation,which was 31%,28% and 11% higher than the control;VB2 improved both the biomass and the L-serine production equally,however didn't influence the distribution of metabolic pathway of L-serine;folate and VB12 could diminish L-serine degradation flux ratio and increase the YP/S and YP/X by accelerating the efficiency of the one carbon units metabolism.The final L-serine productions were 39% and 82% higher than the control.Finally,the fermentation period was 6 h shorter by adding all these vitamins at their optimal doses than by adding biotin only,and the accumulation of L-serine and the biomass were 9.0g/L and 11g/L,which were 70% and 76% higher than the control.
%K L-serine Corynebacterium glutamicum Vitamins Metabolic regulation Optimization<br>L-丝氨酸
%K 谷氨酸棒杆菌
%K 维生素
%K 代谢调控
%K 优化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=105B402371870A82&yid=A732AF04DDA03BB3&vid=DB817633AA4F79B9&iid=94C357A881DFC066&sid=771152D1ADC1C0EB&eid=E514EE58E0E50ECF&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=2&reference_num=19