%0 Journal Article
%T Cloning and expression of SRP19 gene of halophilic archaeon Haloferax volcanii, and purification and analysis of the biological activity of the recombinant protein<br>Hv古细菌SRP19基因的克隆、表达、重组蛋白的纯化及生物学活性的研究
%A HUANG Qiao-jia
%A Christian Zwieb
%A LIN Xu
%A LIN Jian-yin
%A <br>黄俏佳
%A Christian Zwieb
%A 林旭
%A 林建银
%J 中国生物工程杂志
%D 2007
%I 
%X Objective: To construct pET23d-HvSRP19 expression vector for the gene of SRP19 protein of halophilic archaeon Haloferax volcanii(Hv SRP19), and induce it to express in E.coli. Then to purify and analyze the biological activity of its expressed proteins. Methods: The Hv SRP19 full length gene sequences were firstly got from a set of 10 overlapping synthetic short oligonucleotides by de novo recombinant DNA techniques and splicing, and then cloned to pET23d vector. The large expressed products of recombinant plasmid in E.coli BL21(DE3)pLysS were purified by Q-Sepharose ion exchange chromatography, and its biological activity was then analyzed by sucrose density gradient ultra-centrifugation. Results: pET23d-HvSRP19 expression vector was correctly constructed, and found to have a very good expression in E.coli. The purification of the expressed products was confirmed to be successful with an achievement of the protein pure degree up to 95%, and the purified proteins were demonstrated to have the Hv SRP19 biological activity due to it could form complex with Hv SRP RNA. Conclusions:The availability of the Hv SRP19 interaction with Hv SRP RNA was considered to be the initiation of SRP formation and performing function.
%K SRP Halophilic archaeon Haloferax volcanii(Hv) Hv SRP19 Protein induced expression and purification Sucrose density gradient ultra-centrifugation<br>信号识别颗粒
%K Hv古细菌
%K 信号识别颗粒蛋白19
%K 蛋白表达与纯化
%K 密度梯度超速离心
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=2EEF21714C9FB474&yid=A732AF04DDA03BB3&vid=DB817633AA4F79B9&iid=38B194292C032A66&sid=2A8D03AD8076A2E3&eid=EA389574707BDED3&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=11