%0 Journal Article
%T High Cell Density Cultivation of Recombinant Escherichia coli for Production of Fusion Protein Staphylokinase-Hirudin<br>表达重组葡激酶-水蛭素融合蛋白的大肠杆菌工程菌的高密度培养
%A ZHONG Gen-shen
%A SHI Bing-xing
%A WANG Hai-dong
%A JIANG Zhong-hua
%A WU Zu-ze
%A <br>钟根深
%A 石炳兴
%A 王海东
%A 蒋中华
%A 吴祖泽
%J 中国生物工程杂志
%D 2006
%I 
%X Recombinant fusion protein staphylokinase-hirudin has been produced by adopting the dissolved oxygen feed-back fed-batch with the high cell density cultivation strategy in E.coli BL21(DE3). After selection of the strain and the preliminary optimization by flask cultivation, the fermentation was performed with comparing the differences between defined medium and complex medium in 5L fermentor. With the optimization of the cultivated conditions, the recombinant fusion protein staphylokinase-hirudin was over-expressed in E.coli BL21(DE3), and the final cell density reach 115g wet cell weight per liter, the target protein is about 1.1~1.2g/L accounting for more than 30% of the total protein. The culture process in 5L fermentor was performed on 40L fermentor successfully, and indicated that the process was easy scalable.
%K Recombinant Escherichia coli Staphylokinase Hirudin High-cell-density cultivation<br>重组大肠杆菌
%K 葡激酶
%K 水蛭素
%K 高密度培养
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=D2FB63567C10DA27&yid=37904DC365DD7266&vid=96C778EE049EE47D&iid=9CF7A0430CBB2DFD&sid=708DD6B15D2464E8&eid=23CCDDCD68FFCC2F&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=3&reference_num=19