%0 Journal Article
%T A method for quantification of double strand DNA using SYBR Green I dye<br>基于SYBR Green I的双链DNA定量方法
%A LIU Xin
%A XU Gen-ming
%A GUO Jiang-feng
%A DING Xian-feng
%A GAO Xiao-lian
%A <br>刘歆
%A 徐根明
%A 郭江峰
%A 丁先锋
%A 高晓莲
%J 中国生物工程杂志
%D 2008
%I 
%X Abstract An accurate and high-throughput fluorescence based method was developed for quantification of double strand DNA (dsDNA) using SYBR Green I dsDNA-binding dye. A series of diluted genomic DNA and  DNA were mixed with SYBR Green I (4×), and the fluorescence signal was measured with real-time PCR instrument and calibrated with ROX (1×) as reference dye. The performance characteristics of the method were compared with spectrophotometric quantification based on ultraviolet absorption. The new dsDNA quantification using SYBR Green I exhibited linearity over a range of  DNA concentrations from 0.015 to 2 ng/ l and was about 100-times more sensitive and accurate than spectrophotometric quantification. The new dsDNA quantification allowed DNA sample may contain impurities and only required a small quantity of tissue sample. It could be widely used in molecular biological and diagnostic applications as an accurate and high-throughput assay tool.
%K SYBR Green I
%K quantification
%K double strand DNA
%K genomic DNA<br>SYBR
%K Green
%K I
%K 定量
%K 双链DNA
%K 基因组DNA
%K SYBR
%K Green
%K I
%K 双链
%K 定量方法
%K Strand
%K Double
%K Quantification
%K 临床检验
%K 生物学研究
%K 分子
%K 定量要求
%K 生物样本
%K 低纯度
%K 线性关系
%K 荧光强度
%K 范围
%K 荧光定量法
%K 检测限
%K 紫外分光光度法
%K 比较
%K 平行测定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=FFDB779B4CD8B21F14527AAA72C77EFF&yid=67289AFF6305E306&vid=D3E34374A0D77D7F&iid=CA4FD0336C81A37A&sid=E514EE58E0E50ECF&eid=BFE7933E5EEA150D&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=19