%0 Journal Article
%T Inhibition of K-RASAsn12 Expression by Vector-based RNA Interference in Human Pancreatic Cancer Cell Line<br>应用载体介导的RNAi技术抑制胰腺癌细胞K-RASAsn12的表达
%A MENG Fan-jie
%A FU Ze-xian
%A ZHANG Feng
%A LI Bao-dong
%A XIE Shao-jian
%A CAI Jian-hui
%A <br>孟繁杰
%A 付泽娴
%A 张峰
%A 李保东
%A 谢绍建
%A 蔡建辉
%J 中国生物工程杂志
%D 2006
%I 
%X To silence the expression of K-RASAsn12 in human pancreatic cancer cell line by vector-based RNAi(RNA interference) technique,two single-strand DNA sequences encoding mutant-specific shRNA (short haipin RNA) for K-RASAsn12 were synthesized and then inserted into pSilenCircle. The recombinant plasmid was called pSC-K-RASAsn12. According to the same method, pSC-GFP encoding shRNA for GFP was gained. Both recombinant plasmids were transfected into human pacreatic cancer cell line AsPC-1 and BxPC-3. The expression level of K-RASAsn12 was detected by semi-quantitative RT-PCR and Western blot. The result indicated that the recombinant plasmid edcoding mutant-specific shRNA for K-RASAsn12 can inhibit significantly the expression of K-RASAsn12 without affection of wild-type K-RAS(K-RASWT)in Human Pancreatic Cancer Cell Line.
%K RNAi<br>胰腺癌K-RAS
%K 短发夹RNA
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=312E80D8588F8769&yid=37904DC365DD7266&vid=96C778EE049EE47D&iid=E158A972A605785F&sid=7AA74D31F1FF2DCE&eid=869807E2D7BED9EC&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=10