%0 Journal Article
%T Immobilization of Luciferase by Cloning and Expression of Biotinylated Luciferase<br>生物素化荧光素酶的克隆表达及其固定化研究
%A RONG Jing-jing
%A CHEN Zhi-yao
%A ZHOU Guo-hua
%A <br>戎晶晶
%A 陈之遥
%A 周国华
%J 中国生物工程杂志
%D 2007
%I 
%X With purpose of performing biotinylation of firefly luciferase in vivo, we fused a fragment of gene encoding C-terminal 87 amino acids of biotin carboxyl carrier protein (BCCP) of E.coli with luciferase cDNA from firefly Pyrocoelia pectoralis. Biotin was covalently linked to a specific lysine residue of BCCP via catalysis of biotin holoenzyme sythetase in E.coli, and firefly luciferase was biotinylated indirectly as a result of fusion with BCCP. Advantages of specific coupling between biotin and avidin or streptavidin allow firefly luciferase to be immobilized on solid support coated with avidin or streptavidin, so that more flexible and convenient methods of luminescence assay will be available in application. The details of cloning, expression and function of biotinylated luciferase will be discussed in this paper.
%K Luciferase BCCP Biotinylation Fused Expression Enzymatic immobilization<br>荧光素酶
%K 生物素羧基载体蛋白
%K 生物素酰化
%K 融和表达
%K 固定化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=1DD1D93EA876CB4C&yid=A732AF04DDA03BB3&vid=DB817633AA4F79B9&iid=9CF7A0430CBB2DFD&sid=2001E0D53B7B80EC&eid=D997634CFE9B6321&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=20