%0 Journal Article
%T Expressing Purification and Identification of Neuritin Gene in the E.coil<br>人Neuritin在原核表达系统的构建及表达纯化
%A TANG Juan
%A YU Na
%A WU Liang-sheng
%A YANG Lei
%A XIAN Ling-ling
%A HUANG Yan-hong
%A ZHANG Shu-jun
%A HUANG jin
%A <br>唐娟
%A 杨磊
%A 吴亮生
%A 于娜
%A 仙玲玲
%A 黄延红
%A 张树军
%A 黄瑾
%J 中国生物工程杂志
%D 2006
%I 
%X Neuritin is a new neurotrophic factor found recently. In order to identify the function of Neuritin clearly, the coding sequence of human neuritin was amplified by PCR from neuritin cDNA , this fragment digested by NocI and NotI was inserted into pET32a by T4 ligase and transformed into E. coli BL21 then the recombinant plasmid named pET32a-neuritin was constructed successfully . Neuritin was expressed distinctly after inducing by EPTG. The product was identified as neuritin by SDS-PAGE and Western blot analysis . The expression production was purified on Ni2 -NTA column.
%K Human neuritin Prokaryotic expression cloning Purification<br>重组Neuritin
%K 原核表达
%K 纯化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=E90373414F147288&yid=37904DC365DD7266&vid=96C778EE049EE47D&iid=E158A972A605785F&sid=D997634CFE9B6321&eid=771152D1ADC1C0EB&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=2&reference_num=12