%0 Journal Article
%T Purification and Antimicrobial Activity of Lipopeptide Produced by Bacillus natto TK-1 and Its Surfactant Property<br>Bacillus natto TK-1产脂肽的纯化、抑菌活性及其表面活性剂特性
%A CAO Xiao-hong
%A LIAO Zhen-yu
%A WANG Chun-ling
%A HA Zhi-rui
%A YANG Ya-jing
%A LU Mei-fang
%A LI Jian-rong
%A <br>曹小红
%A 廖振宇
%A 王春玲
%A 哈志瑞
%A 杨亚静
%A 鲁梅芳
%A 励建荣
%J 中国生物工程杂志
%D 2008
%I 
%X Lipopeptide biosurfactant from Bacillus natto TK-1 was purified via acidic precipitation, methanol extract and thin-layer chromatography (TLC) system. The surface-active substance migrated at the position of Rf 0.58-0.65 as a single line on the TLC. The critical micelle concentration (CMC) was estimated to be around 115 mg/L and the minimum surface tension of lipopeptide was 30.1 mN/m at a concentration of 512 mg/L. Meanwhile, the anti-adhesion activity of lipopeptide was investigated in vitro. The results indicated that lipopeptide was found to significantly inhibit the adhesion of Salmonella typhimurium, Escherichia coli and Staphylococcus aureaus to 96- well microtiter plates at various concentrations. The highest anti-adhesion activity was exhibited against Salmonella typhimurium with inhibition percentage till 51.9% at 12.8 g/L. The antimicrobial activity of lipopeptide was evaluated against ten microorganisms using disc diffusion method and the lipopeptide showed a broad spectrum of antimicrobial activity. The strongest inhibitory effect was found against Botrytis cinerea and Fusarium moniliforme.
%K Bacillus natto
%K Lipopeptide
%K Surfactin
%K Anti-adhesion
%K Antimicrobial activity<br>Bacillus
%K natto
%K TK-1
%K 脂肽
%K 表面张力
%K 抗粘连
%K 抑菌活性
%K Bacillus
%K natto
%K 脂肽
%K 纯化
%K 抑菌活性
%K 表面活性剂
%K 特性
%K Property
%K Surfactant
%K Lipopeptide
%K Antimicrobial
%K Activity
%K 抑菌能力
%K 灰霉
%K 抑菌谱
%K 考察
%K 平板扩散法
%K 效果
%K 粘附
%K 固体表面
%K 孔板
%K 黄色葡萄球菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=ED042A4FC9DBECA8AAC29C3AF1B1B8C1&yid=67289AFF6305E306&vid=D3E34374A0D77D7F&iid=CA4FD0336C81A37A&sid=1AE5323881A5ECDC&eid=B6DA1AC076E37400&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=18