%0 Journal Article
%T Construction and Expression of Fusion Domains Derived from HIV-1 gp160 and Immunogenicity Analysis of Its Purified Protein<br>HIV-1 gp160多表位嵌合基因的构建及表达蛋白的免疫原性分析
%A YANG Fan
%A LIU Chao-qi
%A LIU Fa-yong
%A YU Feng-hua
%A YANG Chun-xiu
%A HAN Li
%A REN Dong-ming
%A ZHANG Wei
%A YANG Zhu-wei
%A <br>杨凡
%A 刘朝奇
%A 柳发勇
%A 余枫华
%A 杨春秀
%A 韩莉
%A 任东明
%A 张伟
%A 杨祖伟
%J 中国生物工程杂志
%D 2008
%I 
%X Human immunodeficiency virus (HIV) is the etiologic agent of AIDS.Immunogenic epitopes of HIV resides on virus-encoded envelope glycoproteins. To prepare HIV-1 gp160 protein and used it for clinical diagnosis, three gene fragments of HIV-1 env containing highly immunogenic epitopes were amplified by PCR from plasmid of HIV-1 HXB2. The resulting PCR products were linked and cloned into a prokaryotic expression vector pET28a(+) and the accuracy of the inserted fragments was identified by sequencing. To express the HIV-1 gp160 fusion epitopes in E. coli cells and identify fusion protein, the induced protein was checked with SDS-PAGE and Western Blot (WB). The identified HIV positive serum samples were tested by Western Blot (WB) to analysis immunogenicity of the purified protein. The length of the chimeric fragment derived from HIV-1 gp160 was 969bp, and encoded 37kDa amino acid residues. Procaryotic expression plasmid was constructed successfully which can highly effectively express gp160 fusion protein. Recombinant fusion protein was expressed in Eserichia coli BL21(DE3) as an insoluble protein. Procaryotic expression plasmid which can highly effectively express gp160 fusion protein was constructed successfully. The founding and subjects were provided for the research of diagnosis kit.
%K HIV-1gp160
%K fusion gene
%K procaryotic expression
%K immunogenicity
%K western blot<br>HIV-1
%K gp160嵌合基因
%K 蛋白表达
%K 免疫原性
%K 免疫印迹
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=A184B503CA6F68F728CAE299ACF2569D&yid=67289AFF6305E306&vid=D3E34374A0D77D7F&iid=F3090AE9B60B7ED1&sid=7C3A4C1EE6A45749&eid=ECE8E54D6034F642&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=1&reference_num=8