%0 Journal Article
%T Prokaryotic expression and identification of nucleocapsid gene of Canine Distemper Virus CDV-FOX-TA strain from Fox<br>狐源犬瘟热病毒CDV-FOX-TA株核蛋白基因的原核表达及鉴定
%A jianzhongyou jian jiayun
%A <br>简中友
%A 贾贇
%A 王全凯
%A 胡传伟
%A 肇慧君
%A 李叶
%J 中国生物工程杂志
%D 2008
%I 
%X The high conservative sequence of Canine Distemper Virus (CDV) N gene was amplified with RT-PCR, cloned into pMD18-T vector , and then constructed into upstream site of His Tag coding sequence in prokaryotic expression vector pET24b. The N fusion protein was highly expressed when the recombinant plasmid was transformed E coli Rosetta 2(DE3) strain and induced with IPTG. SDS-PAGE and Western blot showed the 15kD expressed products reacted with standard positive serum against CDV. An indirect ELISA demonstrated recombinant expressed protein had good antigencity and can distinguish effectively anti-CDV positive serum and negative serum. The results showed expressed CDV N protein had similarity of antigencity with natural N protein, and can be used as diagnosis antigen. It provided evidence for establishment of an indirect ELISA method for CDV detection.
%K Key words: Canine Distemper Virus
%K nucleocapsid protein
%K prokaryotic expression
%K immunogenicity<br>犬瘟热病毒
%K 核蛋白
%K 原核表达
%K 免疫原性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=C6FC2C845699756180BF5A9640F7C741&yid=67289AFF6305E306&vid=D3E34374A0D77D7F&iid=DF92D298D3FF1E6E&sid=8E6AB9C3EBAAE921&eid=11B4E5CC8CDD3201&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=9