%0 Journal Article
%T Rescue of FMDV from Contransfecting Full Length Infectious RNA of WFL Strain and Eukaryotic Expression Plasmid of FMDV ORF Gene<br>口蹄疫病毒WFL株ORF基因真核表达质粒的构建及病毒的拯救
%A REN Lin-zhu
%A WANG Xing-long
%A CUI Li-jin
%A ZHANG Hui
%A MENG Ke-yin
%A <br>任林柱
%A 王兴龙
%A 崔丽瑾
%A 张辉
%A 孟柯音
%J 中国生物工程杂志
%D 2008
%I 
%X An eukaryotic expression plasmids pEGFP-C1-A3I3,with open reading frame(ORF) of FMDV WFL strain, was constructed based on the pEGFP-C1.The results of transient expression showed that the eukaryotic expression plasmids can be expressed in BHK-21.When cotransfected the plasmid with RNA transcribed from linearized cDNA clone,FMDV antigen and nucleotide acids was detected in cell culture fluid by sandwich ELISA and RT-PCR,respectively.And the virus particles were also found by electron microscope.The results proved that cotransfection of eukaryotic expression plasmid with RNA can improve virus rescuing.And for the first time we successfully rescued FMDV by cotransfecting eukaryotic expression plasmid with RNA transcribed in vitro.
%K foot-and-mouth disease virus
%K open reading frame(ORF)
%K eukaryotic expression
%K reverse genetics
%K rescue<br>口蹄疫病毒
%K 开放性阅读框
%K 真核表达
%K 反向遗传技术
%K 拯救
%K 口蹄疫
%K 病毒
%K 基因组
%K 真核表达质粒
%K Gene
%K FMDV
%K Plasmid
%K Eukaryotic
%K Expression
%K Strain
%K Length
%K 敏感细胞
%K 共转染
%K 粒子
%K 细胞培养液
%K 电镜观察
%K ELISA
%K 夹心
%K 体外转录
%K 发现
%K 结果
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=4ECF58D637E936986A0EB42C3C122ED4&yid=67289AFF6305E306&vid=D3E34374A0D77D7F&iid=E158A972A605785F&sid=DB817633AA4F79B9&eid=4AD960B5AD2D111A&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=12