%0 Journal Article
%T Muitiple target optimization on the fumaric acid production by Rhizopus oryzae based on Desirability function<br>基于Desirability函数法对米根霉发酵制备富马酸的多目标优化
%A Zhang Kun Zhen Gao Shuang Li Yong-Qian Fu He Huang Ping Wei Song-Yang Ji
%A <br>张昆
%A 高振
%A 李霜
%A 付永前
%A 黄和
%A 韦萍
%A 嵇松杨
%J 中国生物工程杂志
%D 2008
%I 
%X Using the strain of Rhizopus oryzae ME-F12, the fermentation conditions for fumaric acid were studied for the purpose of optimizing both fumaric acid production and productivity. An orthogonal experiment was designed to investigate the influence of glucose concentration, urea concentration, seed age and inoculation size. The optimal seed age and inoculation size was determined as 36h and 6ml, respectively. A Desirability function based on response surface analysis was further applied to perform the multiple variable optimization. The best glucose and urea concentration were determined as 132.73g/l and 0.0586g/l, respectively. The predicted value for fumaric acid production, productivity and the Desirability value were 71.42g/l, 0.804g/(l.h) and 0.966, respectively. Verification experiment was carried out on a 5L fermentor. After a 88h fermentation period, 130g/l glucose was converted into 66.5g/l fumaric acid with the productivity of 0.755 g/(l.h). Comparing to the value before optimization, the production and productivity of fumaric acid were increased by 13.9% and 15.8%. Results indicated that both the production and productivity were successfully optimized simultaneously, which laid a solid foundation for the further scale-up.
%K 富马酸
%K 生产速率
%K 优化
%K 响应曲面
%K Desirability函数
%K 函数法
%K 米根霉
%K 发酵制备
%K 富马酸
%K 多目标优化
%K Function
%K Based
%K Rhizopus
%K oryzae
%K Production
%K Fumaric
%K Acid
%K Optimization
%K on
%K Target
%K 工业化
%K 法制
%K 同时优化
%K 效果
%K 验证试验
%K 水平
%K 发酵罐
%K 条件
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=B643AFB1EEB7EC59660B408A3E32A288&yid=67289AFF6305E306&vid=D3E34374A0D77D7F&iid=E158A972A605785F&sid=6AC2A205FBB0EF23&eid=0401E2DB1F51F8DE&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=15