%0 Journal Article
%T Cloning and Expression of Vivrio Cholerae CTB Gene and the Recombinant CTB Protein Activation Assay<br>霍乱弧菌CTB基因的克隆、表达及重组蛋白活性分析
%A ZHANG Guo-guang
%A ZENG Ya-ming
%A LI Dong-xiao
%A ZHANG Hong-xin
%A CHEN Liang
%A <br>张国广
%A 曾雅明
%A 李东霄
%A 张红心
%A 陈亮
%J 中国生物工程杂志
%D 2006
%I 
%X CTB protein possessed mucosal adjuvant immunoactivity. The CTB gene was amplified by PCR method from a strain V. cholerae. The nucleotide sequence of CTB gene was 375 bp and shared 96.0%~99.2% homology with other 6 CTB genes. The recombinant plasmid pTWIN1-CTB transformed E. coli strain BL21(DE3) expressed with 0.8 mmol/L IPTG. The molecular weight of expression products was identical with expectative weight by SDS-PAGE electrophoresis. The CTB fusion proteins mainly assembled inclusion bodies and the outputs of proteins were approximately 20% of the total bacterial proteins. The CTB proteins possessed mucosal immunoactivity by GM1-ELISA assay.
%K Vibrio Cholerae CTB gene Expression of CTB gene GM1-ELISA<br>霍乱弧菌CTB基因
%K 原核表达
%K 粘膜佐剂活性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=88227A0612894EE5&yid=37904DC365DD7266&vid=96C778EE049EE47D&iid=F3090AE9B60B7ED1&sid=FC0714F8D2EB605D&eid=BCA2697F357F2001&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=7