%0 Journal Article
%T Culture of microencapsulated endostatin-CHO cells<br>表达内皮抑素的rCHO细胞的微囊化培养
%A ZHANG Ying
%A WANG Wei
%A L
%A <br>张英
%A 王为
%A 吕国军
%A 于炜婷
%A 郭昕
%A 雄鹰
%A 马小军
%J 中国生物工程杂志
%D 2007
%I 
%X Microencapsulated recombinant cells technology is a novel approach to tumors therapy.It is great importance in encapsulated cells therapy to find a proper way for acquiring seed cells in large-scale in a short time for large scale preparation of biological microcapsule with high cell viability and recombinant protein production.The way and technique to gain adequate seed cells for cell encapsulation with bioreactor culture system were explored.The results showed that the recombinant CHO cells could expand rapidly in the bioreactor in three dimensions and the cell amount reached about 20 times of the initial amount after 10 days culture.The expanded cells could be encapsulated into the microcapsule and the microencapsulated cells could keep high viability and expand rapidly in the bioreactor.In comparison with the cells statically cultured in 24-well plates,a quicker growth and a higher endostatin production of microencapsulated cells were observed.B.Braun bioreactor provided the culture environment with relatively low shear force and good communication among cells in three-dimension,therefore recombinant CHO cells showed a strong ability to expanded in vitro.Large number of seed cells can be obtained rapidly with this culture system,which can meet the requirement of encapsulated cells therapy in both quantity and quality.
%K Microcapsule Seed cell culture Bioreactor Large-scale culture Recombinant CHO cell<br>微胶囊
%K 种子细胞培养
%K 生物反应器
%K 规模化培养
%K rCHO细胞
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=85D8D7E9B2BA8D49&yid=A732AF04DDA03BB3&vid=DB817633AA4F79B9&iid=9CF7A0430CBB2DFD&sid=CA4FD0336C81A37A&eid=DF92D298D3FF1E6E&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=17