%0 Journal Article
%T Constrction and prokaryotic expression of a new anti-HIV targeted toxin<br>抗AIDS新型导向毒素CVN-LP1融合基因的构建及原核表达
%A LIU Yu-sheng
%A JIN Ning-yi
%A LI Chang
%A YU Fang
%A JIN Hong-tao
%A LI Zhen
%A Tong Jing-shan
%A Hu Ning-ning
%A <br>李昌
%A 金宁一
%A 刘玉生
%A 于芳
%A 金洪涛
%A 李臻
%A 佟敬山
%A 胡宁宁
%J 中国生物工程杂志
%D 2007
%I 
%X Objective: To construct prokaryotic expression vector containing CVN-LP1 gene ,and identify the expression of CVN-LP1 protein. Methods: pET-CVN and pET-LP1 was restriction endonuclease digested by EcoRI and HindIII, and the CVN gene was inserted into pET-LP1 plasmid. The vector was transformed into BL21(DE3) to construct a prokaryotic expression system. The expressed product was identified by SDS-PAGE and Western blot assay. Results: The recombinant plasmid pET-CVN-LP1 was constructed, restriction endonuclease digestion and PCR identification proved that the CVN was correctly cloned into vector pET-LP1. SDS-PAGE and Western-blot analysis showed that CVN-LP1 was successfully expressed in E.coli BL21(DE3) ,The relative molecular mass (Mr) of the expression protein was 20kDa,according with the predicted Mr value. And the expression yield was about 49.58% of total bacterial protein. Conclusion: The successful expression of CVN-LP1 gene in E.coli provides a support for further study of the anti-HIV agent.
%K Cyanovirin-N
%K LuffinP1<br>核糖体失活蛋白
%K 原核表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=445135EF77C16497&yid=A732AF04DDA03BB3&vid=DB817633AA4F79B9&iid=38B194292C032A66&sid=8C83C265AD318E34&eid=DBF54A8E2A721A6D&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=1&reference_num=9