%0 Journal Article
%T Adherence of Neisseria gonorrhoeae to hCD46 expressing COS-1 cells<br>淋病奈瑟菌对hCD46基因转染COS-1细胞的黏附
%A LI Guo-cai
%A WANG Jin-song
%A ZHU Li-tian
%A WANG Xiao-hong
%A JIAO Hong-mei
%A PAN Xing-yuan
%A JI Ming-chun
%A <br>李国才
%A 王劲松
%A 朱立天
%A 王晓红
%A 焦红梅
%A 潘兴元
%A 季明春
%J 中国生物工程杂志
%D 2008
%I 
%X To study the role of hCD46 in mediating the specific adhesion by N. gonorrhoeae to its human host, an hCD46 minigene including the promoter and cDNA was synthesized by using ligation-mediated PCR, and cloned into vector pcDNA3.1 replacing the original CMV promoter. The resultant recombinant vector pCD46 was transfected into COS-1 cells. The effective expression of hCD46 on the membrane of COS-1 cells was detected by indrect immunofluorscence assay; Western blot revealed that the molecular weight of the recombinant hCD46 was correct. The hCD46 expressing COS-1-46 cells were sorted by FACS and could be adhered by piliated N. gonorrhoeae stain YZ1228 revealed by bacteria binding assay. These data indicate that hCD46 is a kind of crucial pilus receptor that mediates the host specific adhesion by N. gonorrhoeae, and the hCD46 minigene, pCD46, can be used to develop transgenic mice model for the study of gonococcal diseases.
%K Neisseria gonorrhoeae
%K human CD46
%K adhesion<br>淋病奈瑟菌
%K 人CD46
%K COS-1细胞
%K 黏附
%K 淋病奈瑟菌
%K 基因转染
%K 膜细胞
%K 黏附性
%K Cells
%K Human
%K Neisseria
%K gonorrhoeae
%K 小鼠模型
%K 转基因
%K 菌感染
%K 受体
%K 临床分离株
%K 阳性
%K 显示
%K 实验
%K 细菌黏附
%K 转染细胞
%K 流式细胞术
%K 分子量
%K 有效表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=4DC673FE62FB3553829B06AFD1A7331B&yid=67289AFF6305E306&vid=D3E34374A0D77D7F&iid=E158A972A605785F&sid=42425781F0B1C26E&eid=2001E0D53B7B80EC&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=17