%0 Journal Article
%T Study on the in vitro cleavage activity of an artificial HCV-specific M1GS ibozyme<br>HCV特异性M1GS核酶的构建及体外切割活性研究
%A ZHANG Wen-jun
%A NING Rong
%A ZHANG Xin
%A LI Hong-zhi
%A <br>张文军
%A 宁容
%A 张欣
%A 李红枝
%J 中国生物工程杂志
%D 2008
%I 
%X In this study, a sequence-specific M1GS ribozyme (M1GS-HCV/C20) has been successfully constructed by covalently linking an oligonucleotide (guide sequence, GS) to the 3' terminus of M1 RNA, the catalytic subunit of RNase P from Escherichia coli. The engineered ribozyme is targeted to the most conservative sequence (5'UTR) of HCV genome, and can effectively cleave the substrate RNA segment in vitro. Undoubtly, the M1GS-HCV/C20 we got would be a useful experimental material to futher study its cleavage activity in vivo, and can be even used for evaluating its anti-viral effect in the animal model. It was believed that this study would markedly facilitate the research of a general gene targeting agent for anti-HCV applications, and layed the foundation for developing a new nucleic acid drug and a novel strategy of anti-HCV therapy.
%K 核酶P
%K 引导序列
%K 丙型肝炎病毒
%K 5'非编码区
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=82C989BE8DDE047DFDABEB582F48D0D9&yid=67289AFF6305E306&vid=D3E34374A0D77D7F&iid=9CF7A0430CBB2DFD&sid=A4FA325EA800C820&eid=89F76E117E9BDB76&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=10