%0 Journal Article
%T Two-Step MS-PCR combined with ELISA Method for the detection of Drug Resistance Mutations in HIV-1 RT Gene<br>用两步MS-PCR结合ELISA检测HIV-1逆转录酶基因的耐药性突变
%A HE Hong-qiu
%A CHENG Shao-hui
%A LIU Bin
%A CHEN Wei-zu
%A WANG Cun-xin
%A <br>何红秋
%A 程绍辉
%A 刘斌
%A 陈慰祖
%A 王存新
%J 中国生物工程杂志
%D 2006
%I 
%X Highly Active Anti-Retroviral Therapy (HAART) has effectively inhibited the prevalence of HIV-1 and reduced the death rate caused by AIDS. In recent years,the emergence of resistance-conferring RT gene mutations in HIV-1 strains has become the major reason for HAART failure. The detection of drug resistance is important for the HAART regimen choice and novel drug development. A novel assay for the detection of HIV-1 RT drug resistance mutations was developed. HIV-1 drug resistance and wild strains in B subtypes were investigated using Two-Step Mutagenically-Separated PCR (MS-PCR),and point mutations including M41L,K70R,K103N,Y181C,T215F were detected. A longer mutant type primer was designed,using microplates hybridization and ELISA technique to detect several point mutations within a mixed mutant-wild type population. The results indicate that the Two-Step MS-PCR is as sensitive and specific as that in the traditional MS-PCR and MS-PCR combined with ELISA can give a good P/N quotient with better sensitivity,low cost,relatively less time consumption and high-throughput screening. It will be used in clinic usage for the detection of HIV-1 drug resistance mutations as well as other point mutations.
%K ELISA
%K HIV-1<br>两步MS-PCR
%K 逆转录酶基因
%K 耐药性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=2A96FA19D6E64059&yid=37904DC365DD7266&vid=96C778EE049EE47D&iid=5D311CA918CA9A03&sid=ECE8E54D6034F642&eid=D997634CFE9B6321&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=18