%0 Journal Article %T Optimization of lipase production conditions by Geotrichum candidum Y162 using single factor-response surface methodology
单因子-响应面法优化白地霉Y162产脂肪酶条件 %A YAN Jin-yong %A YANG Jiang-ke %A YAN Yun-jun %A
阎金勇 %A 杨江科 %A 闫云君 %J 中国生物工程杂志 %D 2007 %I %X The fermentation conditions of lipase production by Geotrichum candidum Y162 were optimized. Initially, the most suitable carbon olive oil, nitrogen source soybean flour and NH4Cl, salt BaCl2 and MgCl2 were selected according to single factorial experiments respectively. Based on the result, screening methodology Plackett-Burman design was used to evaluate the effects of twelve factors related to lipase production and three statistically significant factors olive oil, BaCl2 and NH4Cl were selected. The path of steepest ascent was used to approach the optimal region of lipase production subsequently. Then, the optimal combined concentration for maximum enzyme activity were further optimized by response surface methodology and determined as follows: olive oil 2.35%, BaCl2 0.36%,and NH4Cl 4.69%.The optimization of culture conditions of G.candidum Y162 led to a 2.25-fold increase in lipase production relative to initial result 14.16 U/ml, which indicate that single factor in combination with response surface methodology is an effective method for optimization of lipase production conditions by G.candidum Y162. %K Lipase Geotrichum candidum Y162 Plackett-Burman design Response surface methodology Optimization
脂肪酶 %K 白地霉Y162 %K Plackett-Burman设计 %K 响应面法 %K 优化 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=AE15ABF6DBCFCAE0&yid=A732AF04DDA03BB3&vid=DB817633AA4F79B9&iid=5D311CA918CA9A03&sid=95D537AC89B28832&eid=68D88C2FCF9C3098&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=1&reference_num=11