%0 Journal Article
%T A Novel Biocatalyst and its optimized process for Preparing L-theanine<br>L-茶氨酸新型酶法制备及其催化工艺优化
%A LI Jia-you
%A GUO Li-yun
%A JIAO Qing-cai
%A <br>李加友
%A 郭丽芸
%A 焦庆才
%J 中国生物工程杂志
%D 2007
%I 
%X L-theanine (γ-glutamylethylamide) is the main free amino acid component of tea and its favorable physiological effects on mammals have been reported. An enzymatic method for optically pure L-theanine production with a new L-aminoacylases-production fungi Cunnighamella echinulata 9980 was developed. The optimum conditions for enzymatic catalysis were at pH 6.5 with 50 mmol/L N-Acyl-DLtheanine and 40 mmol/L CoCl2. After 12-h incubation at 50℃,22.5 mmol/L L-theanine was obtained, the conversion rate against N-Acyl-L-theanine being 90%. Cunnighamella echinulata and the aminoacylase were applied in preparation of L-theanine.
%K Cunnighamella echinulata
%K L-theanine
%K N-Acyl-DL-Theanine
%K Cunnighamella echinulata
%K L-aminoacylase
%K Optical resolution<br>L-茶氨酸
%K N-乙酰-DL-茶氨酸
%K L-氨基酰化酶
%K 光学拆分
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=6321D2742E68F897AA6BB72B6676801B&yid=A732AF04DDA03BB3&vid=DB817633AA4F79B9&iid=F3090AE9B60B7ED1&sid=16D8618C6164A3ED&eid=7C3A4C1EE6A45749&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=14