%0 Journal Article
%T Noninvasive Determination of Intracellular Free Mg2+ by 31P and 19F NMR: A Review
31P和19F NMR在细胞内游离镁离子测定中的研究
%A QIAO Juan
%A HUANG Rong-qing
%A XIAO Bing-kun
%A LUO Chuan-huan
%A ZHANG Hong-yi
%A
乔娟
%A 黄荣清
%A 肖炳坤
%A 骆传环
%A 张红医
%J 波谱学杂志
%D 2005
%I
%X Magnesium ion (Mg~2+) plays important roles in many biochemical reactions occurring in cells, and determination of intracellular free Mg~2+ concentration is essential for understanding the functioning of intact cells Nuclear magnetic resonance (NMR), a spectroscopic technique that measures magnetic nuclei and their interactions with the environment, has gained considerable success and popularity in studying metal ions in vivo ~31P and ~19F NMR can be used to measure intracellular concentration of free Mg~2+, and these techniques has the advantage of noninvasiveness so that free Mg~2+ in the intracellular environment can be observed in essentially unperturbed living systems In this review, the theories and applications of ~31P and ~19F NMR for determining intracellular concentration of free Mg~2+ are discussed:
%K 31P NMR
%K 19F NMR
游离镁离子
%K 无损伤测定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=47EA7CFDDEBB28E0&jid=1A3C8C6E452BD1AF5A6B6B99BA3989C9&aid=6269DD8C6EDE7C38&yid=2DD7160C83D0ACED&vid=BC12EA701C895178&iid=CA4FD0336C81A37A&sid=A63576421B012172&eid=CDEBD1ACE0A4C1C1&journal_id=1000-4556&journal_name=波谱学杂志&referenced_num=0&reference_num=30