%0 Journal Article
%T Novel Isotope Labeling Strategies for Protein Solution NMR Spectroscopy: A Review<br>核磁共振研究中蛋白质样品的同位素标记策略
%A XUAN Jin-song
%A WANG Jin-feng
%A <br>宣劲松
%A 王金凤
%J 波谱学杂志
%D 2008
%I 
%X Protein isotope labeling is crucial for NMR studies of the three-dimensional structure and dynamics of proteins in solution. Protein expression systems and isotope labeling have been widely used, both of which have been proven useful. However, as the field progresses, the studies on protein with NMR are becoming increasingly difficult with these conventional techniques. Recently, some new isotope protein labeling strategies and new protein expression systems have emerged as tools for studying large proteins by solution NMR. In this paper, these novel protein isotope labeling methods and alternative protein expression systems are reviewed.
%K NMR
%K isotope labeling
%K protein expression systems
%K 3D solution structures of proteins<br>核磁共振技术
%K 同位素标记技术
%K 蛋白质表达系统
%K 蛋白质溶液三维结构
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=47EA7CFDDEBB28E0&jid=1A3C8C6E452BD1AF5A6B6B99BA3989C9&aid=40FF0DFF5A18FB4244657643451B1202&yid=67289AFF6305E306&vid=C5154311167311FE&iid=38B194292C032A66&sid=8D75AD3BD0D1BCC5&eid=A48DE16C07AAAB06&journal_id=1000-4556&journal_name=波谱学杂志&referenced_num=0&reference_num=28