%0 Journal Article
%T Response of respiratory rate and reactive oxygen species scavenging enzyme activity in seed mitochondria of Clausena lansium dehydration and its ecological significance<br>黄皮种子线粒体呼吸速率和活性氧清除酶活性对脱水的响应及其生态学意义
%A WANG Wei-Qing
%A CHENG Hong-Yan
%A LIU Shu-Jun
%A SONG Song-Quan
%A <br>王伟青
%A 程红焱
%A 刘树君
%A 宋松泉
%J 植物生态学报
%D 2012
%I Editorial Office of Chinese Journal of Plant Ecology
%X Aims Recalcitrant seeds shed at relatively high water contents and metabolism activities are sensitive to dehydration. However, the mechanism for desiccation sensitivity of recalcitrant seeds is unclear. Here, we used the recalcitrant Chinese wampee (Clausena lansium) seeds as materials to investigate the relationship between desiccation sensitivity and the change in respiratory rate and reactive oxygen species (ROS) scavenging enzyme activity of mitochondria. Methods We dehydrated Chinese wampee seeds at 25–28 °C and 72%–80% relative humidity for 0, 4, 7 and 10 days then assayed the survival of seeds and axes, changes in respiratory rate of axes and cotyledons, activity of cytochrome c oxidase (CCO), integrity of outer mitochondrial membrane, pathways of CCO and alternative oxidase (AOX) and activities of ROS scavenging enzymes in mitochondria of axis and cotyledon. Important findings With the loss of water content, survival of seeds and axes gradually decreased, and desiccation sensitivity of seeds was greater than that of axes. Respiratory rate and integrity of outer mitochondrial membrane of axes and cotyledons decreased with dehydration. Respiratory rate of CCO pathway in mitochondria of axis and cotyledon and of AOX pathway in mitochondria of cotyledon increased in the initial phase of dehydration and then decreased, and that of AOX pathway in mitochondria of axis significantly decreased with dehydration. Activities of superoxide dismutase (SOD), ascorbate peroxidase (APX) and glutathione reductase (GR) in axis mitochondria and those of APX in cotyledon mitochondria decreased with dehydration, and those of dehydroascorbate reductase (DHAR) in axis mitochondria and of SOD, DHAR and GR in cotyledon mitochondria increased in the initial phase and then decreased. Our data indicate that desiccation sensitivity of Chinese wampee seeds is closely related to decreases in respiratory rate and reactive oxygen species (ROS) scavenging enzyme activity of mitochondria and also to long-term adaptation to the tropical/subtropical habitat.
%K Clausena lansium seed
%K cytochrome c oxidase and alternative oxidase pathway
%K dehydration injury of seed and axis
%K mitochondria reactive oxygen species scavenging enzymes
%K respiratory rate<br>黄皮种子
%K 细胞色素c氧化酶和交替氧化酶途径
%K 种子和胚轴的脱水伤害
%K 线粒体活性氧清除酶
%K 呼吸速率
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=2F2173CCFF292BF447DC2681EA33BBAE&aid=3EB2C91B216F2588FC8FFF3B6DA34AE4&yid=99E9153A83D4CB11&vid=933658645952ED9F&iid=5D311CA918CA9A03&sid=971ECAFE8682845B&eid=D698D0190A84C2BD&journal_id=1005-264X&journal_name=植物生态学报&referenced_num=0&reference_num=0