%0 Journal Article
%T ¦Â-Catenin Is a Positive Regulator of Estrogen Receptor-¦Á Function in Breast Cancer Cells
%A Nibedita Gupta
%A Fee Schmitt
%A Sina Grebhardt
%A Doris Mayer
%J Cancers
%D 2011
%I MDPI AG
%R 10.3390/cancers3032990
%X Estrogen receptor-alpha (ER¦Á) is a key factor in the development of breast cancer in humans. The expression and activity of ER¦Á is regulated by a multitude of intracellular and extracellular signals. Here we show a cross-talk between ¦Â-catenin and ER¦Á in human breast cancer cells. Knockdown of ¦Â-catenin by RNAi resulted in significant reduction of ER¦Á mRNA and/or protein levels in MCF-7, T-47D, and BT-474 breast cancer cells and in significant reduction of estradiol-induced expression of the ER¦Á target genes pS2 and GREB1. In addition ¦Â-catenin silencing resulted in significant decrease of growth of MCF-7 cells both in the absence and presence of estradiol. ¦Â-catenin and ER¦Á could not be co-immunoprecipitated by ER¦Á antibodies from lysates of E2-treated or untreated cells suggesting lack of direct physical interaction. It is concluded that ¦Â-catenin is a positive regulator of ER¦Á mRNA and protein expression.
%K ¦Â-catenin
%K estrogen£¿receptor
%K cross-talk
%K gene expression
%K transcriptional activity
%K breast cancer cell
%U http://www.mdpi.com/2072-6694/3/3/2990