%0 Journal Article
%T Production of maleylpyruvate and fumarylpyruvate<br>顺丁烯二酸-单酰丙酮酸及反丁烯二酸-单酰丙酮酸的制备
%A LIU Dong-qi
%A LIU Hong
%A ZHOU Ning-yi
%A <br>刘冬啟
%A 刘虹
%A 周宁一
%J 浙江大学学报(农业与生命科学版)
%D 2005
%I Zhejiang University Press
%X Gentisate 1,2-dioxygenase (GDO,EC1.14.13) from Klebsiella pneumoniae M5a1 successfully converted gentisate to maleylpyruvate. This biotransformation was performed by using genetically engineered E.coli strain carrying a gene encoding gentisate 1,2-dioxygenase, and the maleylpyruvate synthesized by this enzyme attained a purity as high as 97.7%. Purity of fumarylpyruvate obtained by acidifying the resulting maleylpyruvate with meta-phosphoric acid was 96.4%. This study thus established a method which is suitable for the production of maleylpyruvate and fumarlpyruvate.
%K maleylpyruvate
%K fumarylpyruvate
%K entisate 1
%K 2-dioxygenase
%K Klebsiella pneumoniae: M5a1
%K preparation<br>顺丁烯二酸-单酰丙酮酸
%K 反丁烯二酸-单酰丙酮酸
%K 龙胆酸1
%K 2-双加氧酶
%K 肺炎克氏杆菌M5a1
%K 制备
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=298920A27C9BAA22346FCA384240FAA4&jid=466C05D88593309B3EB5CF34361E6426&aid=5174E9B9C60EA45E&yid=2DD7160C83D0ACED&vid=4AD960B5AD2D111A&iid=38B194292C032A66&sid=DFEE4E8C33C95CEF&eid=8ED630AD8C61FAE8&journal_id=1008-9209&journal_name=浙江大学学报(农业与生命科学版)&referenced_num=0&reference_num=11