%0 Journal Article
%T Cloning of coat protein gene of Grapevine fanleaf virus Hangzhou isolate and construction of its plant expression vector<br>葡萄扇叶病毒外壳蛋白基因克隆和植物表达载体的构建
%A 李红叶
%A 陈力耕
%A 周雪平
%J 浙江大学学报(农业与生命科学版)
%D 2002
%I Zhejiang University Press
%X 用RT-PCR法从葡萄扇叶病毒杭州分离物(GFLV-H)基因组RNA中扩增了该病毒分离物的外壳蛋白基因cDNA片段,并克隆到质粒pGEM-T-easyVector.序列分析结果表明,该基因含有1515个核苷酸,编码504个氨基酸,其核苷酸和推导的氨基酸与GFLV法国分离物F13的同源性分别为88%和95%.目前该基因已成功地克隆到植物表达载体pBI121,经三亲交配导入到根癌农杆菌(Agrobacteriumtumefaciens)LBA4404中.
%K Grapevine fanleaf virus
%K gene cloning
%K sequence analysis<br>葡萄扇叶病毒
%K 基因克隆
%K 序列分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=298920A27C9BAA22346FCA384240FAA4&jid=466C05D88593309B3EB5CF34361E6426&aid=C6EA041E2BE49541&yid=C3ACC247184A22C1&vid=D3E34374A0D77D7F&iid=B31275AF3241DB2D&sid=6C62BFE34266FA92&eid=421C41B8FD4D3EBA&journal_id=1008-9209&journal_name=浙江大学学报(农业与生命科学版)&referenced_num=1&reference_num=6