%0 Journal Article
%T RAPD analysis of the genetic specificity of WHBE rabbit<br>WHBE兔遗传特异性的RAPD分析
%A CHEN Min-li
%A ZHAO Wei-Chun
%A YING Hua-zhong
%A WANG De-jun
%A XU Xiao-ping
%A <br>陈民利
%A 赵伟春
%A 应华忠
%A 王德军
%A 徐孝平
%J 浙江大学学报(农业与生命科学版)
%D 2005
%I Zhejiang University Press
%X Genetic relationship among White Hair and Black Eyes (WHBE) rabbits, Japanese big ear rabbits and New Zealand rabbits were analyzed with randomly amplified polymorphic DNA (RAPD) technique. Ten random primers were used to amplify genomic DNA of rabbits with PCR methods. On the basis of preliminary experiments, eight primers were selected to analyze the genetic variation among 3 rabbit lines. 107 amplified fragments were detected by electrophoresis on agarose gel. Among them, 32 fragments were polymorphic, accounting for 29.91% of the whole. It was showed that the RAPD polymorphisms among rabbit strain lines were abundant. The results of the statistical analysis of RAPD bands of rabbits showed that the genetic similarity between WHBE rabbits and Japanese big ear rabbit was 0.7948, which was the highest among 3 rabbit strain lines. Four primers among 10 random primers could not only amplify the common DNA bands of 3 rabbit strain lines, but also amplify the different characteristic bands of them. The amplified band patterns showed that there are both genetic similarities and genetic variations among WHBE rabbit, Japanese big ear rabbit and New Zealand rabbit. Thus, the RAPD technique could be used as a method to monitor genetic relationship among different strains and different individuals of the same strain of rabbits.
%K WHBE rabbit
%K randomly amplified polymorphic DNA (RAPD)
%K genetic variation<br>WHBE兔
%K 随机扩增多态DNA技术(RAPD)
%K 遗传差异
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=298920A27C9BAA22346FCA384240FAA4&jid=466C05D88593309B3EB5CF34361E6426&aid=556A9D2C4FFF5A14&yid=2DD7160C83D0ACED&vid=4AD960B5AD2D111A&iid=E158A972A605785F&sid=8C267C8DC97FEEEF&eid=E3C3E274D87A8C16&journal_id=1008-9209&journal_name=浙江大学学报(农业与生命科学版)&referenced_num=19&reference_num=8