%0 Journal Article
%T Cloning and comparative analysis of rDNA ITS2 and 5' terminal sequence of COⅠ gene of Frankliniella occidentalis<br>西花蓟马（Frankliniella occidentalis） rDNA ITS2和COⅠ基因5’末端序列的克隆与比较分析
%A WU Xiao-yun
%A CHENG Xiao-fei
%A ZHANG Zhong-kai
%A GUI Fu-rong
%A LI Zheng-yue
%A <br>武晓云
%A 程晓非
%A 张仲凯
%A 桂富荣
%A 李正跃
%J 浙江大学学报(农业与生命科学版)
%D 2009
%I Zhejiang University Press
%X Using PCR technique, both rDNA ITS2 and 5' terminal sequence of COⅠgenes of Frankliniella occidentalis were cloned and sequenced. Based on nucleotide sequences obtained in the present and from GenBank, the mutation type, nucleotide diversity, haplotype diversity and average intra-species genetic distance of rDNA ITS2 and COⅠ were analyzed and compared. Results show that COⅠ was a better candidate gene for detecting intra-specific polymorphisms, and to characterize genetic diversity;F. occidentalis may consist of two (or more) strains or cryptic (sub) species, and F. occidentalis population in China consisted of both greenhouse strain and lupin strain, with greenhouse strain as majority. Based on the knowledge thus far, we presumed that the first invaded area of F. occidentalis was Yunnan Province, and from there this exotic invasive insect dispersed to other parts of China, such as Beijing and Harbin. These results provide a basis for understanding the genetic diversity, strain composition and invasion mechanism of F. occidentalis in China.
%K Frankliniella occidentalis
%K genetic analysis
%K strain composition
%K invasion mechanism<br>西花蓟马
%K 遗传分析
%K 株系组成
%K 入侵机制
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=298920A27C9BAA22346FCA384240FAA4&jid=466C05D88593309B3EB5CF34361E6426&aid=3EFCCED8D92D52A3350FCE33ED1D66DF&yid=DE12191FBD62783C&vid=6209D9E8050195F5&iid=E158A972A605785F&sid=26AEEED215BE97D0&eid=F7496043B4F12D75&journal_id=1008-9209&journal_name=浙江大学学报(农业与生命科学版)&referenced_num=2&reference_num=23