%0 Journal Article
%T Construction of eukaryotic expression vector of Interleukin-2 gene from Xiaoshan Chicken and its expression in Vero cells<br>萧山鸡IL-2基因真核表达载体构建及其在Vero细胞中的表达
%A XIE Rong-hui
%A FANG Wei-huan
%A LI Jian-rong
%A LU Mi-jia
%A YU Lian
%A <br>谢荣辉
%A 方维焕
%A 李建荣
%A 卢觅佳
%A 于涟
%J 浙江大学学报(农业与生命科学版)
%D 2003
%I Zhejiang University Press
%X In previous study IL-2 gene of Xiaoshan Chicken has been cloned by our laboratory, and in this study the cDNA of IL-2 was amplified and inserted into the eukaryotic expression vector pCI. The resulted recombinant plasmid pCI-IL-2 was transformed by electroporation into an attenuated Salmonella typhimurium strain ZJ111, which was then used to transfect Vero cells. DNA extracted from the transfected Vero cells showed a positive signal as hybridized with DIG-labeled probe in a DNA blotting assay, which revealed that the IL-2 gene was integrated into the genome of Vero cells. Specific yellow-green fluorescence was visual in indirect immunofluorescence assay with FITC-labeled goat anti-rabbit IgG. The 14.5 kD and 18.5 kD bands correspond to IL-2 protein from lysate of Vero cells was identified by SDS-PAGE and Western blotting, indicating that in Vero cells IL-2 was expressed successfully with the characteristics of immunological reactivity.
%K attenuated Salmonella typhimurium
%K Xiaoshan Chicken
%K Interleukin-2
%K eukaryotic expression<br>减毒沙门氏菌
%K 萧山鸡
%K 白细胞介素2
%K 真核表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=298920A27C9BAA22346FCA384240FAA4&jid=466C05D88593309B3EB5CF34361E6426&aid=AB4AEA0E4B207D5C&yid=D43C4A19B2EE3C0A&vid=771469D9D58C34FF&iid=B31275AF3241DB2D&sid=95F0D0DE8CD6F434&eid=BBA8B1249CDAA6CE&journal_id=1008-9209&journal_name=浙江大学学报(农业与生命科学版)&referenced_num=0&reference_num=18