%0 Journal Article %T Characteristics and 3,5-dichlorocatechol 1,2 -dioxygenase gene location of Pseudomonas sp GT241-1
降解2,4-二氯酚的假单胞菌GT241-1的特性及其3,5-二氯儿茶酚1,2-双加氧酶基因定位 %A ZHONG Wen-hui %A HE Guo-qing %A ZHENG Ping %A SUN Ming %A YU Zi-niu %A
钟文辉 %A 何国庆 %A 郑平 %A 孙明 %A 喻子牛 %J 浙江大学学报(农业与生命科学版) %D 2001 %I Zhejiang University Press %X A 2,4-dichlorophenol degrading bacterium strain, designated as GT241-1, was isolated from the soil around drainage area of several factories which produced 2,4-dichlorophenol. It was identified as Pseudomonas sp. Strain GT241-1 can decompose 91% of 2,4-dichlorophenol with original concentration of 90 mg/L within 48 hours. GC/MS analysis revealed that the strain can degrade 2,4-dichlorophenol completely, without accumulation of intermediate metabolite. Heat treatment at 38℃ made 1.9% of GT241-1 strains lose its 2,4-dichlorophenol degrading ability. Strain GT241-1 has two big plasmids. Southern blot results showed that the 3,5-dichlorocatechol 1,2-dioxygenase gene of strain GT241-1 locates on its about 11kb EcoRI/XbaI fragment. %K 假单胞菌GT241-1 %K 2 %K 4-二氯酚 %K 3 %K 5-二氯儿茶酚 %K 1 %K 2-双加氧酶基因 %K 基因定位 %K 降解 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=298920A27C9BAA22346FCA384240FAA4&jid=466C05D88593309B3EB5CF34361E6426&aid=7DA77C0DD62671E0&yid=14E7EF987E4155E6&vid=DB817633AA4F79B9&iid=38B194292C032A66&sid=1A363081E1FF7014&eid=4E6F5C60B72D9B1C&journal_id=1008-9209&journal_name=浙江大学学报(农业与生命科学版)&referenced_num=3&reference_num=7