%0 Journal Article
%T Phylogenetically clustering of rhizobial reference strains based on numbers and location of their 23S rRNA genes<br>根瘤菌参比菌株23S rRNA基因数量和定位及其系统发育群
%A ZHENG Jun-fang
%A LIU Gui-rong
%A LIU Shu-lin
%A HE Jun-qi
%A <br>郑君芳
%A 刘桂荣
%A 刘树林
%A 贺俊崎
%J 浙江大学学报(农业与生命科学版)
%D 2008
%I Zhejiang University Press
%X To clarify phylogenetic relationships among rhizobial reference strains,the numbers and location of their 23S rRNA genes were analyzed by pulsed field gel electrophoresis(PFGE) after I-CeuI cleavage.According to the similarity in the numbers and location of their 23S rRNA genes,rhizobial reference strains were grouped into 19 phylogenetic clusters.Of which,13 clusters were consistent with taxonomic groupings(taxa) in genera level,but 6 clusters not.The clustering method based on the numbers and location of rhizobial 23S rRNA genes also divided the rhizobia belonging to the same taxa into different phylogenetic clusters.These results suggest that identification of genomic features using PFGE after DNA cleavage with I-CeuI can further characterize current rhizobial taxa into different phylogenetic clusters with a high genetic homogeneity inside clusters.
%K rhizobia
%K 23S rRNA gene
%K phylogenetic analysis
%K pulsed field gel electrophoresis(PFGE)
%K I-CeuI<br>根瘤菌
%K 23S
%K rRNA基因
%K 系统发育分析
%K 脉冲场电泳
%K I-CeuI
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=298920A27C9BAA22346FCA384240FAA4&jid=466C05D88593309B3EB5CF34361E6426&aid=9BE39EE32CF5A0767CB5621AF32F6075&yid=67289AFF6305E306&vid=339D79302DF62549&iid=94C357A881DFC066&sid=BE5DBC360CD4FFB9&eid=D397660E39E3E461&journal_id=1008-9209&journal_name=浙江大学学报(农业与生命科学版)&referenced_num=0&reference_num=21