%0 Journal Article
%T Study on antifungal active substance of Bacillus subtilis F-2<br>枯草芽孢杆菌F-2抗植物病原真菌活性物质的研究
%A LU Xiao-cheng
%A ZHAO Yu-hua
%A FANG Ping
%A <br>鲁小城
%A 赵宇华
%A 方萍
%J 浙江大学学报(农业与生命科学版)
%D 2007
%I Zhejiang University Press
%X Effects of Bacillus subtilis F-1 and F-2 on inhibiting the growth of Fusarium oxysporum f.sp. cucumerinum (F.o.c) were studied by a co-culture experiment. The results showed that the growth of F.o.c could be strongly inhibited by the specific metabolites of Bacillus subtilis F-2, but not by Bacillus subtilis F-1. It was guessed by LC-MS determination that the possible molecular weights of the specific metabolites were 1477.0, 1489.1 and 1505.1. It was further found by MALDI-TOF MS that there were two groups of homologues with a difference of 14 Da in molecular weights in the extract of Bacillus subtilis F-2. In the first group, there were 5 kinds of homologues with the molecular weights of 1462.8, 1476.8, 1490.8, 1504.8 and 1518.9, respectively, which were identified as lipopeptide antibiotics Fengycin B(C_ 14-18 ) by the method of mass fingerprinting. In the second group, the molecular weights of 5 kinds of homologues were 1432.8, 1446.8, 1460.8, 1474.8 and 1488.9, respectively, which were estimated as the isomer of Fengycin A(C_ 14-18 ) as only 2 Da difference in molecular weights.
%K LC-MS
%K MALDI-TOF MS<br>枯草芽孢杆菌
%K 脂肽抗生素
%K 质量指纹谱技术
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=298920A27C9BAA22346FCA384240FAA4&jid=466C05D88593309B3EB5CF34361E6426&aid=959DEB75E7B40FAC&yid=A732AF04DDA03BB3&vid=27746BCEEE58E9DC&iid=CA4FD0336C81A37A&sid=339D79302DF62549&eid=7C3A4C1EE6A45749&journal_id=1008-9209&journal_name=浙江大学学报(农业与生命科学版)&referenced_num=2&reference_num=8