%0 Journal Article
%T Establishment of infectious bursal disease virus(IBDV) reverse genetics system using T7 RNA polymerase<br>基于T7 RNA聚合酶的传染性法氏囊病病毒反向遗传系统的建立
%A WEI Yong-wei
%A ZHANG Xiao-dong
%A HOU Xian-hong
%A XU Hong
%A YU Lian
%A <br>魏永伟
%A 章晓栋
%A 侯贤宏
%A 徐弘
%A 于涟
%J 浙江大学学报(农业与生命科学版)
%D 2008
%I Zhejiang University Press
%X In order to establish the reverse genetics of IBDV,the sequences of T7 promoter and ribozyme HDV were introduced into the 5' and 3' terminals of segments A and B of IBDV genome using PCR.Then the segments,which containing promoter and ribozyme HDV sequences,were cloned into PT-Pluc vector and named as PT-mA and PT-mB, respectively.Both PT-mA and PT-mB were co-transfected into vero cells which were infected by recombinant vaccinia virus vTF7-3(vTF7-3 can express the T7 RNA polymerase in vero cell) for 1 hour.The surveying of cytopathic effect(CPE),indirect immunofluorescence test and molecular marker detection confirmed the succeeding of the rescuing of IBDV.
%K IBDV
%K reverse genetics
%K T7 RNA polymerase<br>IBDV
%K 反向遗传
%K T7RNA聚合酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=298920A27C9BAA22346FCA384240FAA4&jid=466C05D88593309B3EB5CF34361E6426&aid=25570CBF8FBF9570CF038F051F66B02F&yid=67289AFF6305E306&vid=339D79302DF62549&iid=B31275AF3241DB2D&sid=3B2BF7AC5674E8E2&eid=D647AF4730396036&journal_id=1008-9209&journal_name=浙江大学学报(农业与生命科学版)&referenced_num=0&reference_num=24