%0 Journal Article
%T Influencing factors on maize transgene process mediated by Agrobacterium.<br>农杆菌介导玉米转基因的影响因素研究
%A BAI Yun-Feng
%A WANG Guo-Ying
%A GOU Ming-Yue
%A ZHAO Jin-Feng
%A DONG Zhi-Gang
%A <br>白云凤
%A 王国英
%A 苟明月
%A 赵晋锋
%A 董志刚
%J 中国生态农业学报
%D 2006
%I 
%X The effects of explant selection,high osmotic treatment,co-culture with Agrobacterium,screening of transformed plants by selecting medium on the callus growth and transformation efficiency were studied in the process of maize transformation mediated by Agrobacterium.The results show that the growth rate and transformation efficiency of immature embryos derived from a vigorous ear are higher than those from other ears.High osmotic treatment and co-culture with Agrobacterium and herbicide screening impede the callus growth.The solutions are put forward.
%K Maize transgene
%K Agrobacterium
%K Herbicide
%K Transformation efficiency<br>玉米转基因
%K 农杆菌介导
%K 除草剂
%K 转化效率
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=1A8B5357F0EF07B8&jid=AB3ABF502E2E1FF39F0E3B80164C9031&aid=02A999DD36596CB1&yid=37904DC365DD7266&vid=F3583C8E78166B9E&iid=38B194292C032A66&sid=DABEF202280E7EF1&eid=A58CF3BAE79427D0&journal_id=1671-3990&journal_name=中国生态农业学报&referenced_num=1&reference_num=8