%0 Journal Article
%T Inhibitory effects of strawberry extracts on animal fatty acid synthase<br>草莓提取物对脂肪酸合酶及脂肪细胞的抑制作用
%A LIU Xiao-Xin
%A TIAN Wei-Xi
%A MA Xiao-Feng
%A <br>刘晓鑫
%A 田维熙
%A 马晓丰
%J 中国科学院研究生院学报
%D 2010
%I 
%X Fatty acid synthase (FAS) has been considered as a potential new therapeutic target for obesity. In the present study we show that the components of both strawberry ( Fragaria ananassa Duch) fruit dreg and strawberry leaves that extracted with 50% ethanol and partitioned by ethyl acetate are potent FAS inhibitors. The ethyl acetate fractions of strawberry fruit dregs (SF-AE) show noncompetitive inhibition with the substrate Mal-CoA, while the ethyl acetate fractions of strawberry leaves (SL-AE) show competitive inhibition with Ac-CoA. Based on the HPLC-MS analysis, we conclude that ellagic acid and quercetin are the efficient components. SF-AE and SL-AE could reduce lipids accumulation in 3T3-L1 preadipocyte with dose-dependence. These results may be of great value for application of strawberry extracts to obesity prevention and cure.
%K fatty acid synthase
%K strawberry
%K inhibitor
%K kinetic assay
%K anti-obesity<br>脂肪酸合酶
%K 草莓
%K 抑制剂
%K 抑制动力学
%K 减肥
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=B5EDD921F3D863E289B22F36E70174A7007B5F5E43D63598017D41BB67247657&cid=B47B31F6349F979B&jid=67CDFDECD959936E166E0F72DE972847&aid=9EF107A3210EF76815C03EAC2DF28CCC&yid=140ECF96957D60B2&vid=DB817633AA4F79B9&iid=B31275AF3241DB2D&sid=B5D9C773C430C13C&eid=583C993D4E08F5CE&journal_id=1002-1175&journal_name=中国科学院研究生院学报&referenced_num=0&reference_num=25