%0 Journal Article
%T Molecular Pre-identification of Interspecies Hybrids in Prunus<br>樱桃属种间杂种的早期分子鉴定
%A WANG Hong-Xia
%A YE Nai-Hao
%A LI Wen-Sheng
%A ZHANG Kai-Chun
%A SHU Huai-Rui
%A <br>王鸿霞
%A 叶乃好
%A 李文生
%A 张开春
%A 束怀瑞
%J 中国科学院研究生院学报
%D 2006
%I 
%X Incompatibility of interspecies cross between sweet cherry and Cerasus tomentosa was overcome by successive hand pollination, and the new germplasm G3 was obtained by using embryo culture technology to overcome the embryo abortion of distance hybridization. The G3 S-genotype was identified in seedling using AS-PCR, and the self-incompatibility gene of the parent was used as the molecular marker to validate the pedigree of hybrid G3. The RAPD analysis further demonstrated that G3 share characteristic RAPD fragments of both parents. This result suggested that AS-PCR was an effective and credible method to identify the progeny of interspecies hybrid in Prunus. And the cross could be a promising way to enhance the genetic background. DNA molecular marker realized the identification and pre-selection in the seedling stage. So the developing breeding program would be accelerated by the new way.
%K distance hybrid
%K embryo culture
%K AS-PCR
%K RAPD<br>远缘杂交
%K 胚培养
%K 等位基因特异性PCR
%K 随机引物扩增多态性DNA
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=B5EDD921F3D863E289B22F36E70174A7007B5F5E43D63598017D41BB67247657&cid=B47B31F6349F979B&jid=67CDFDECD959936E166E0F72DE972847&aid=034B15252AEF95CD&yid=37904DC365DD7266&vid=EA389574707BDED3&iid=94C357A881DFC066&sid=93661EC4C0CCEA67&eid=DEE640F0CDC9D495&journal_id=1002-1175&journal_name=中国科学院研究生院学报&referenced_num=3&reference_num=8