%0 Journal Article
%T Transformation of Lettuce with FMDV Epitopes Fused Gene Mediated by Agrobacterium<br>口蹄疫抗原决定簇融合基因转化生菜的研究
%A DENG Xiao-Li
%A CHANG Jing-Ling
%A HE Jie
%A HE Guang-Cun
%A <br>邓小莉
%A 常景玲
%A 贺杰
%A 何光存
%J 植物科学学报
%D 2006
%I 
%X Plant-derived vaccine is becoming one focus of plant gene engineering,which has advantages such as easy production,low cost,convenient and safe use.In this experiment,the fused gene O_(21)-O_(14)-A_(21) containing both type O and A FMDV epitopes was introduced into lettuce mediated by agrobacterium.The hygromycin-resistant lettuce calli were obtained by screening on the medium containing antibiotic.Fifty six hygromycin-resistant lettuce seedlings were regenerated.The results of PCR and PCR-Southern blotting indicated that the fusion gene were successfully integrated into the genomes some of transformed lettuce plants.Expression of O_(21)-O_(14)-A_(21) gene in transformed lettuce plants was proved by RT-PCR(analyses).
%K Foot-and-mouth disease
%K Epitopes
%K Genetic transformation
%K Lettuce(Lactuca sativa var
%K capatata L
%K )
%K Gene expression<br>口蹄疫
%K 抗原决定簇
%K 遗传转化
%K 生菜
%K 基因表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=9973DE552EA54506F7AABD4780345ED2&aid=0C4BE5BD5DEDEF0A&yid=37904DC365DD7266&vid=B91E8C6D6FE990DB&iid=94C357A881DFC066&sid=DA4893B5F9885621&eid=03436AC72A659ACA&journal_id=1000-470X&journal_name=武汉植物学研究&referenced_num=0&reference_num=8