%0 Journal Article %T Transformation of Lettuce with FMDV Epitopes Fused Gene Mediated by Agrobacterium
口蹄疫抗原决定簇融合基因转化生菜的研究 %A DENG Xiao-Li %A CHANG Jing-Ling %A HE Jie %A HE Guang-Cun %A
邓小莉 %A 常景玲 %A 贺杰 %A 何光存 %J 植物科学学报 %D 2006 %I %X Plant-derived vaccine is becoming one focus of plant gene engineering,which has advantages such as easy production,low cost,convenient and safe use.In this experiment,the fused gene O_(21)-O_(14)-A_(21) containing both type O and A FMDV epitopes was introduced into lettuce mediated by agrobacterium.The hygromycin-resistant lettuce calli were obtained by screening on the medium containing antibiotic.Fifty six hygromycin-resistant lettuce seedlings were regenerated.The results of PCR and PCR-Southern blotting indicated that the fusion gene were successfully integrated into the genomes some of transformed lettuce plants.Expression of O_(21)-O_(14)-A_(21) gene in transformed lettuce plants was proved by RT-PCR(analyses). %K Foot-and-mouth disease %K Epitopes %K Genetic transformation %K Lettuce(Lactuca sativa var %K capatata L %K ) %K Gene expression
口蹄疫 %K 抗原决定簇 %K 遗传转化 %K 生菜 %K 基因表达 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=9973DE552EA54506F7AABD4780345ED2&aid=0C4BE5BD5DEDEF0A&yid=37904DC365DD7266&vid=B91E8C6D6FE990DB&iid=94C357A881DFC066&sid=DA4893B5F9885621&eid=03436AC72A659ACA&journal_id=1000-470X&journal_name=武汉植物学研究&referenced_num=0&reference_num=8