%0 Journal Article
%T A High-frequency in vivo-in vitro Experimental System for Embryogenesis Study<br>一个高频率的活体-离体胚胎发生研究系统
%A HE Yu-Chi
%A CHEN Jun-Yi
%A GUO Jing-Ze
%A SUN Meng-Xiang
%A YANG Hong-Yuan
%A <br>何玉池
%A 陈俊义
%A 郭荆哲
%A 孙蒙祥
%A 杨弘远
%J 植物科学学报
%D 2006
%I 
%X An in vivo-in vitro experimental system was set up,in which zygotes could develop into fertile plants through normal embryogenesis with high frequency by means of ovule culture.Some key developmental events of embryogenesis,such as directional expansion,asymmetrical first division,suspensor formation and degeneration as well as embryo differentiation,occurred as that in in planta embryogenesis in the experimental condition.No morphological difference was observed between embryos gained from ovule culture and embryos grown in planta,indicating that the in vitro ovule culture system phenocopies the in planta developmental program of embryogenesis.This system is simple and stable,and therefore can be a useful tool for the study of embryogenesis mechanism.
%K Ovule culture
%K Tobacco(Nicotiana tabacum)
%K Embryogenesis<br>胚珠培养
%K 烟草
%K 胚胎发生
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=9973DE552EA54506F7AABD4780345ED2&aid=1BC544DEE1633BA3&yid=37904DC365DD7266&vid=96C778EE049EE47D&iid=CA4FD0336C81A37A&sid=B31275AF3241DB2D&eid=708DD6B15D2464E8&journal_id=1000-470X&journal_name=武汉植物学研究&referenced_num=0&reference_num=15