%0 Journal Article
%T Response of Protective Enzyme in Callus of Barnyard Grass under Oxidizing Stress Induced by Polyacetylene Compound
多炔化合物氧化胁迫下稗草细胞保护酶系的反应
%A WAN Shu-Qing~
%A YANG Shu-Juan~
%A JIANG Zhi-Sheng~
%A SHANG Zhi-Zhen~
%A LIU Zhun~ South China Agricultural University
%A Guangzhou
%A China
%A Institute of Element-Organic Chemistry
%A Nankai University
%A Tianjin
%A China
%A
万树青
%A 杨淑娟
%A 蒋志胜
%A 尚稚珍
%A 刘准
%J 植物科学学报
%D 2004
%I
%X The callus of barnyard grass (Echinochloa crusgalli)was treated with compound 5 (1-phenyl-4-(3,4-methylenedioxy)-phenyl-diacetylene) under UV-A irradiation for 3 h, which forms an inner environment of oxidizing stress in the cells. Exploiting the method of bioche-mistry, we assay the activity of several protective enzymes in the oxidizing stress. The results showed that compound 5 can stimulate the activity of glutathione-S-transferase(GST), glutathione peroxidase (GSH-Px) and peroxidase(POD) and inhibited the activity of superoxide dismutase (SOD) under UV-A. The activities of GST, GSH-Px, and POD under light were higher than those under non-light. The enhanced percentages of GST, GSH-Px, and POD at the concentration of 10 mg/L under light were 10.47%, 113.68% and 166.68%, but SOD was inhibited at 1 mg/L and 10 mg/L, the inhibiting percentage was 50.25% and 76.46%, respectively. The relationship between compound 5 oxidization and anti-oxidization was discussed in the paper.
%K 1-phenyl-4-(3
%K 4-methylenedioxy)-phenyl-diacetylene
%K Echinochloa crusgalli
%K Protective enzyme
1-苯基-4-(3,4-亚甲二氧)-苯基丁二炔
%K 稗草
%K 保护酶系
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=9973DE552EA54506F7AABD4780345ED2&aid=8A50D22C24965202&yid=D0E58B75BFD8E51C&vid=BC12EA701C895178&iid=E158A972A605785F&sid=9DC563A0FEFC04F9&eid=302684F6FB4B24FF&journal_id=1000-470X&journal_name=武汉植物学研究&referenced_num=0&reference_num=15